MCQ on Microbiology, Sterilization of culture media Part - 4

 

Microbiology MCQ (Quiz-4)  

Sterilization of culture media

Multiple choice questions with answer key

S. Y. B. PHARM Sem - III  (UNIT - 3)  

AS PER PCI SYLLABUS

1. Salts of heavy metals used as disinfectants are

  A) Mercurochrome  
B) Thiomersal
C) Phenyl mercury nitrate
D) All of these

... Answer is : D) All of these

2. The principle involved in the streak plate method is

A) Dilution
B) Isolation
C) Streaking
D) Separation

... Answer is : A) Dilution

3. Spores of actinomycetes are very sensitive, killed at room temperature of

A) 43 oC for 30 min.
B) 52 oC for 30 min.
C) 65 oC for 30 min.
D) 70 oC for 30 min.

... Answer is : C) 65 oC for 30 min.

4. A common laboratory method of cultivating anaerobic micro-organisms is

A) Pyrogallic acid over the cotton
B) Gas pack system
C) Brewer jar system
D) None of these

... Answer is : A) Pyrogallic acid over the cotton

5. The micro-organisms grow at high salinity are

A) Halophiles
B) Osmophiles
C) Both A and B
D) None of these

... Answer is : C) Both A and B

6. Wilson and Blair medium is used for isolation of .

A) Shigella shigae
B) Vibrio cholerae
C) Staphylococci
D) Salmonella typhosa

... Answer is : D) Salmonella typhosa

7. Shigella was first isolated by

A) Schmitz
B) Sonnei
C) Robert Koch
D) Shiga

... Answer is : B) Sonnei

8. Kauffmann white scheme is used to detect

A) E.coli
B) Salmonella spp.
C) Salmonella spp.
D) None of these

... Answer is : B) Salmonella spp.

9. C.diphtheriae requires

A) PDA medium
B) LJ medium
C) Mac Conkey’s medium
D) Potassium tellurite medium

... Answer is : D) Potassium tellurite medium

10. Lepra bacillus is best cultured on

A) Liver of guinea pig
B) Armadillo’s brain
C) Foot pad of mice
D) All of the above

... Answer is : C) Foot pad of mice

11. Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are

A) Sub-cultures
B) Stab cultures
C) Stock cultures
D) None of these

... Answer is : B) Stab cultures

12. Culture media for fungi are

A) Sabouraud’s agar
B) Czapekdox agar
C) Potato dextrose agar (PDA)
D) All of the above

... Answer is : D) All of the above

13. The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures

A) Thermoduric
B) Extreme thermophiles
C) Thermophiles
D) Facultative thermophiles

... Answer is : A) Thermoduric

14. Alkaliphiles grow at pH value between

A) 7 to 12
B) 1 to 11
C) 6 to 9
D) 1 to 6

... Answer is : A) 7 to 12

15. Non-lactose fermenting colonies seen on Mac Conkey’s medium are

A) Klebsiella pneumoniae
B) Shigella shigae
C) Salmonella typhi
D) Escherichia coli

... Answer is : C) Salmonella typhi

16. Laboratory diagnosis of enteric fever is based on

A) Widal test
B) Blood culture
C) Urine and stool culture
D) All of the above

... Answer is : D) All of the above

17. Which of the following are gas producing Salmonella?

A) S. enteritidis
B) S. typhimurium
C) S. typhi
D) S. cholerasuis

... Answer is : A) S. enteritidis

18. On Mac Conkey’s medium Esch. Coli forms

A) Pink coloured colonies
B) Greenish pigmentation
C) Medusa head appearance
D) Colorless colonies

... Answer is : A) Pink coloured colonies

19. Culture medium for Mycobacterium tuberculosis

A) PDA medium
B) Wilson blair medium
C) L J medium
D) Mac Conkey’s medium

... Answer is : C) L J medium

20. Culture medium for clostridia spp.

A) Mac Conkey’s medium
B) Robertson’s cooked meat medium
C) 76 Lower stein Jensen’s medium
D) None of these

... Answer is : B) Robertson’s cooked meat medium