MCQ on Biotechnology


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Multiple choice questions with answers


1. Restriction enzymes were discovered by

(a)  Smith and Nathans

(b)  Alexander Fleming

(c)  Berg

(d)  None

2. Bacteria protect themselves from viruses by fragmenting viral DNA with

(a)  Ligase

(b)  Endonuclease

(c)  Exonuclease

(d)  Gyrase

 

3. Klenow fragment is derived from

(a)  DNA Ligase

(b)  DNA Pol-I

(c)  DNA Pol-II

(d)  Reverse Transcriptase

 

4. Southern blotting is

(a)  Attachment of probes to DNA fragments

(b)  Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet

(c)  Comparison of DNA fragments to two sources

(d)  Transfer of DNA fragments to electrophoretic gel from cellulose membrane

 

 

5. ELISA is

(a)  Using radiolabelled second antibody

(b)  Usage of RBCs

(c)  Using complement-mediated cell lysis

(d)  Addition of substrate that is converted into a coloured end product

 

6. The Golden Rice variety is rich in

(a)  Vitamin C

(b)  Î’-carotene and ferritin

(c)  Biotin

(d)  Lysine

 

7. The DNA fragments have sticky ends due to

(a)  Endonuclease

(b)  Unpaired bases

(c)  Calcium ions

(d)  Free methylation

 

8. Plasmids are used as cloning vectors for which of the following reasons?

(a)  Can be multiplied in culture

(b)  Self-replication in bacterial cells

(c)  Can be multiplied in laboratories with the help of enzymes

(d)  Replicate freely outside bacterial cells

9. In tissue culture of parenchyma, mitosis is accelerated in the presence of ________.

(a) Auxin

(b) Cytokinin

(c) Gibberellin

(d) Both auxin and cytokinin


10. The vaccines prepared through recombinant DNA technology are  

(a)  Third generation vaccines

(b)  First-generation vaccines

(c)  Second-generation vaccines

(d)  None

11. Which is a genetically modified crop?

(a)  Bt-cotton

(b)  Bt-brinjal

(c)  Golden rice

(d)  All

12. PCR technique was invented by

(a)  Karry Mullis

(b)  Boyer

(c)  Sanger

(d)  Cohn

13. The first transgenic plant to be produced is

(a)  Brinjal

(b)  Tobacco

(c)  Rice

(d)  Cotton


14. RNA interference helps in

(a)  Cell proliferation

(b)  Micropropagation

(c)  Cell defense

(d)  Cell differentiation

15. Excision and insertion of a gene is called

(a)  Biotechnology

(b)  Genetic engineering

(c)  Cytogenetics

(d)  Gene therapy


16. The expression of a transgene in the target tissue is identified by a

(a)  Transgene

(b)  Promoter

(c)  Enhancer

(d)  Reporter

17. ———– is used as a vector for cloning into higher organisms

(a)  Retrovirus

(b)  Baculovirus

(c)  Salmonella typhimurium

(d)  Rhizopus nigricans


18. Process used for amplification or multiplication of DNA in DNA fingerprinting is

(a) polymerase chain reaction
(b) southern blotting
(c) northern blotting
(d) none of these.

19. Enzyme ‘Taq polymerase’ used in PCR, has been isolated from bacterium
(a) Agrobacterium tumefaciens
(b) Thermus aquaticus
(c) Streptomyces albus
(d) Escherichia coli


20.Which one of the following is not a correct match ?
(a) Tumour inducing – Ti plasmid
(b) DNA probe – Identifies the desired DNA
(c) PCR – DNA staining
(d) Agarose – Seaweeds


21. The correct sequence of different steps of polymerase chain reaction is
(a) annealing → denaturation → extension
(b) denaturation → extension → annealing
(c) denaturation → annealing → extension
(d) extension → denaturation → annealing.

22. Which of the following is required to perform polymerase chain reaction ?
(a) Primers, dNTPs and DNA polymerase
(b) DNA, CaCl2 and nuclease
(c) Mg + 2, DNA
(d) Both (a) and (c)


23. Which of the following is not used to transfer the recombinant DNA into the host ?
(a) Micro-injection method
(b) Gene gun method
(c) Bioreactors
(d) Disarmed pathogen vectors


24. An enzyme catalysing the removal of nucleotides from the ends of DNA is
(a) endonuclease
(b) exonuclease
(c) DNA ligase
(d) Hind II.

25. The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as
(a) transduction
(b) conjugation
(c) transformation
(d) translation.

1. a

2. b

3. b

4. b

5. d

6. b

7. b

8. b

9. d

10. a

11.d

12. a

13. c

14. c

15.b

16. d

17. a

18. a

19. b

20. c

21.c

22. d

23. c

24. b

25. a


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