MCQ Test on Ion Exchange Chromatography
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Multiple choice question with answers
1. Which sentence is true about batch method ?
A. It is
multiple step process.
B. It is not
used for preparation of the demineralized water.
C. In this
process more than two containers.
D. This is
single step process.
2.
Which method are used for preparing of demineralized water?
A. Gas
Chromatography
B. Batch
method ( ion exchange)
C. Mass
sepctroscopy
D.
Complexometric Titration
3.
Which is not application of ion Exchange ?
A. It is used
for softening of water.
B. It is used
for demineralization of water.
C. It is used
for separation of similar ion in one sample.
D. It is
used in preformulation
4.
Which is application of demineralized water ?
A.
Biological studies
B. conductance
experiment
C. A and B
D. None of
the above
5.
Which is not ion exchange technique ?
A. Batch
method
B. Column
method
C. Paper
Chromatography
D. A and B
6. Ion exchange
capacity of a resin is dependent on :- (GATE 2002)
A. the
total molecular weight of the resin
B. length
of the ion exchange resin
C. the
total number of ion active groups
D. solubility
of the ion exchange resins
7. Ion exchange
chromatography is the method of choice for the separation of- (Gate1988)
A. Metals
B. Sugars
C. Fatty
acid
D. Sterols
8. which one of the
following chromatographic technique is most suitable for separating
glu-Cosamine from glucuronic acid ? (Gate1996)
A. Affinity
chromatography
B. lon
exchange
C. Molecular
exclusion
D. Hydrophobic
chromatography
9. In reverse phase
chromatography, the stationary phase is made
A. non-polar
B. polar
C. either
non-polar or polar
D. none
of these
10. Ion exchange
chromatography is based on the
A. electrostatic
attraction
B. electrical
mobility of ionic species
C. adsorption
chromatography
D. partition
chromatography
11.
Chromatography is generally used for the separation of mixtures based on the
differences in their
A. Shape
B. Size
C. Structure
D. All of Above
12. Ion-exchange
chromatography is used for the separation of
A. polar
molecules
B. non
polar molecules
C. both
of above
D. none
of above
13. Which polymer is
often used for matrix materials in Affinity chromatography?
A. Agarose
B. Cellulose
C. Dextrose
D. All
of Above
14. Which
chromatographic technique depends on the highly specific interactions between
pairs of biological materials such as enzyme-substrate?
A. Adsorption
chromatography
B. Ion
–exchange chromatography
C. Affinity
chromatography
D. Gel-permeation
chromatography
15. Protein
purification refers to the
A. Purification
of proteins
B. Separation
of proteins from other biomolecules
C. Separation
of a particular protein from other contaminating proteins
D. all
of these
16. Primary steps in
protein purification includes
A. Homogenization
B. Differential
centrifugation
C. Solubilisation
D. All
of These
17. The use of insulin
hormone to purify its receptor is an example of
A. Ion
exchange chromatography
B. Affinity
chromatography
C. Gel
filtration chromatography
D. Ligand
mediated chromatography
18. In anion exchange
chromatography,
A. the
column contains negatively charged beads where positively charged proteins bind
B. the
column contains positively charged beads where negatively charged proteins bind
C. the
column contains both positive and negatively charged beads where proteins bind
depending on their net charge
D. all
of these
19. A combination of
paper chromatography and electrophoresis involves
A. partition
chromatography
B. electrical
mobility of the ionic species
C. both
(1) and (2)
D. none
of these
20. Ion exchange
chromatography is based on the
A. electrostatic
attraction
B. electrical
mobility of ionic species
C. adsorption
chromatography
D. partition
chromatography
21. The general
expression for the appearance of a solute in an effluent is (where V is the
elution volume of a substance , V0 void volume, kD distribution constant and Vi
internal water volume)
A. V
= V0 + kDVi
B. V
= V0/Vi
C. V
= V0 – kDVi
D. None
of the above
22. In gel filtration
chromatography, separation of proteins are based on their
A. size
and net charge
B. size
and shape
C. size
and specific affinity
D. shape
and net charge
23. Which of the
following separation method is suited for a protein sample with large
differences in molecular mass
A. dialysis
B. salting
out process
C. density
gradient centrifugation
D. rate
zonal centrifugation
24. Salting out process
involves
A. precipitation
of proteins using ammonium sulphate
B. precipitation
of proteins using copper sulphate
C. precipitation
of proteins using sodium chloride
D. none
of these
25. Protein separation
techniques are often based on the following properties except
A. solubility
of the protein
B. viscosity
of the protein
C. charge
of protein
D. specific
binding affinity of the protein
|
1. D |
2.
B |
3.
D |
4.
C |
5.
C |
6.
C |
|
7.
A |
8.
B |
9.
A |
10.
A |
11.
D |
12.
A |
|
13.
D |
14.
C |
15.
C |
16.
D |
17.
B |
18.
B |
|
19.
C |
20.
A |
21.
A |
22.
B |
23.
D |
24.
A |
|
25.
B |
|
|
|
|
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