MCQ on Biotechnology 



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Multiple choice questions with Answers


1. Who is the father of genetic engineering ?
(a) Steward Linn
(b) Stanley Cohen
(c) Paul Berg
(d) Kary Mullis


2. Plasmid used to construct the first recombinant DNA was isolated from which bacterium species ?
(a) Escherichia coli
(b) Salmonelia typhimurium
(c) Agrobacterium tumefaciens
(d) Thermus aquaticus

 

3. The term ‘molecular scissors’ refers to
(a) recombinant DNA
(b) restriction enzymes
(c) Taq polymerase
(d) palindromic nucleotide sequences.


4. The term ’chemical knife’ refers to
(a) polymerases
(b) endonucleases
(c) ribonucleases
(d) cellulases.

5. One of the key factors, which makes the plasmid the vector in genetic engineering is
(a) its resistance to antibiotics
(b) its resistance to restriction enzymes
(c) its ability to carry a foreign gene
(d) its ability to cause infection in the host.

6. The term ’recombinant DNA’ refers to
(a) DNA of the host cell
(b) DNA with a piece of foreign DNA
(c) DNA with selectable marker
(d) DNA with more than one recognition sites.

7. The term ’chimeric DNA’ refers to
(a) DNA with overhanging stretches
(b) DNA with palindromic sequence
(c) a recombinant DNA
(d) molecular scissors.

8. Which of the following is not a tool of genetic engineering ?
(a) Cloning vector
(b) Restriction enzyme
(c) Foreign DNA
(d) GMO


9. In agarose gel electrophoresis, DNA molecules are
separated on the basis of their
(a) charge only
(b) size only
(c) charge to size ratio
(d) all of the above.

10. Which of the following is not required in the preparation of a recombinant DNA molecule ?
(a) Restriction endonuclease
(b) DNA ligase
(c) DNA fragments
(d) E. coli


11. While isolating DNA from bacteria, which of the following enzymes is not used ?
(a) Lysozyme
(b) Ribonuclease
(c) Deoxyribonuclease
(d) Protease

12. Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________
a) non-specific, directly
b) non-specific, indirectly or directly
c) specific, directly
d) specific, indirectly


13. Which of the following is not suitable if the PCR product is non-specific?
a) Adjusting the concentration of magnesium ions
b) Increasing annealing temperature
c) Using touchdown PCR
d) Using inverse PCR

14.The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved.
a) True
b) False


15. How many approaches are there in order to clone the complete genome?
a) 1
b) 2
c) 3
d) 4

16. The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________
a) amplification
b) polymerase chain reaction (PCR)
c) translation
d) microarrays


17. Which of the following is a characteristic of Taq polymerase?
a) It is an RNA polymerase
b) It is heat stable
c) It is obtained from thermophilic bacterium and can be grown in the laboratory below a temperature of 75 degrees
d) It is used in cellular synthesis processes and the optimum temperature is at least 90 degrees

18. Who is known as the father of tissue culture?

(a) Bonner

(b) LaibachC

(c) Haberlandt

(d) Gautheret


19. The production of secondary metabolites requires the use of ________.

(a) Meristem

(b) Protoplast

(c) Axillary buds

(d) Cell suspension

20.The pair of hormones required for a callus to differentiate are________.

(a) Ethylene and Auxin

(b) Auxin and cytokinin

(c) Auxin and Abscisic acid

(d) Cytokinin and gibberellin


21. The ability of the component cells of callus to form a whole plant is known as

(a)   redifferentiation

(b)   dedifferentiation

(c)    either (a) or (b)

(d)   none of these

22. What is Dimethyl sulfoxide used for?

(a) A gelling agent

(b) Cryoprotectant

(c) Chelating agent

(d) An Alkylating agent


23. The formation of embryoids from the pollen grains in the tissue culture medium is due to ________.

(a) Organogenesis

(b) Test tube culture

(c) Double fertilization

(d) Cellular totipotency

24. Somaclonal variations are the ones

(a)               Caused by mutagens

(b)               Produce during tissue culture

(c)                Caused by gamma rays

(d)               Induced during sexual embryogeny

25.  Totipotency refers to ___________.

(a) Development of fruits from flowers in a culture

(b) Development of an organ from a cell in a culture medium

(c) Flowering in a culture medium

(d) All of the above


1. c

2. b

3. b

4. b

5. c

6. b

7. c

8. d

9.b

10. d

11. c

12.c

13. d

14. a

15. b

16. a

17. b

18. c

19. d

20. b

21.a

22. b

23. d

24. b

25.b


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